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XBP-1 Schematic ( PDF Datasheet ) - MSD

Teilenummer XBP-1
Beschreibung Cell Signal
Hersteller MSD
Logo MSD Logo 




Gesamt 2 Seiten
XBP-1 Datasheet, Funktion
MSD® XBP-1 Kit
For quantitative determination in human, mouse, and rat whole cell lysate samples
Alzheimer’s Disease
BioProcess
Cardiac
Cell Signaling
Clinical Immunology
Cytokines
Growth Factors
Hypoxia
Immunogenicity
Inflammation
Metabolic
Oncology
Toxicology
Vascular
Catalog Numbers
XBP-1 Kit
Kit Size Catalog #
1 plate
K150QKD-1
5 plates K150QKD-2
25 plates K150QKD-4
1. XBP-1
2. BSA blocked
3. BSA blocked
4. BSA blocked
X-box binding protein 1 (XBP-1) is a basic-region, leucine zipper type transcription factor that belongs to the CREB/ATF family and mediates one
of the three arms of unfolded protein response (UPR) adaptation to endoplasmic reticulum (ER) stressors. The accumulation of unfolded or
misfolded proteins in the ER is a threat to cell survival and results in a condition known as ER-stress.1,2 To overcome this stress, the ER initiates
specific signaling pathways encompassed by the ER stress response.3 Among these are translational attenuation, upregulation of ER chaperone
proteins and proteins that facilitate folding, activation of NFkB signaling, and, as a last resort, the induction of apoptosis.4-6 Under basal conditions,
XBP-1 is ubiquitously expressed as a 33 kDa XBP-1u isoform. The initiation of ER stress activates the endonuclease activity of the transmembrane
kinase and endoribonuclease inositol-requiring enzyme-1 (IRE-1) in an unconventional cleavage and re-ligation reaction producing an active 52
kDa splice-variant form of XBP-1 known as XBP-1s.5 XBP-1s transcriptionally regulates the production of additional ER chaperones and secretory
proteins to maintain the function of the organelle and the health of the cell.6 XBP-1 is involved in the response of many diseases linked to ER stress
(ischemia, neurodegeneration, cancer, diabetes), which makes this an attractive marker for many therapeutic areas. The MSD XBP-1 assay is
available on 96-well, 4-spot plates. This datasheet outlines the performance of the assay.
Ordering Information
MSD Customer Service
Phone: 1-301-947-2085
Fax: 1-301-990-2776
Email: CustomerService@
mesoscale.com
Scientific Support
Phone: 1-301-947-2025
Email: ScientificSupport@
mesoscale.com
Typical Data
Representative results for the XBP-1 Kit are illustrated below. The signal and ratio values provided are examples; individual results will vary
depending upon the samples tested.
This assay was developed using recombinant human XBP-1 protein (data shown below) as well as with the nuclear fraction of rat hepatoma cells
treated with the ER stress-inducing agents, thapsigargin and tunicamycin (data not shown). This assay recognizes both the spliced and non-spliced
XBP-1 isoforms referred to in the introduction. Recombinant protein or nuclear cell lysate fractions were added to MULTI-SPOT®, 4-spot plates
coated with anti-XBP-1 antibody on one of the four spatially distinct electrodes in each well. XBP-1 was detected with anti-XBP-1 antibody
conjugated with MSD SULFO-TAG.
500000
Company Address
MESO SCALE DISCOVERY®
A division of
Meso Scale Diagnostics, LLC.
1601 Research Boulevard
Rockville, MD 20850-3173 USA
www.mesoscale.com®
400000
300000
200000
100000
0
For Research Use Only.
Not for use in
diagnostic procedures.
Recombinant Human XBP-1 (ng/mL)
Figure 1: Sample data generated with the XBP-1 Kit. Increased signal is observed with the titration of recombinant human XBP-1 protein.





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