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PDF SC-375 Data sheet ( Hoja de datos )

Número de pieza SC-375
Descripción K-20
Fabricantes Santa Cruz Biotechnology 
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SANTA CRUZ BIOTECHNOLOGY, INC.
www.DataSheet4U.com
Gγ 3 (K-20): sc-375
The Power to Question
BACKGROUND
Heterotrimeric G proteins function to relay information from cell surface
receptors to intracellular effectors. Each of a very broad range of receptors
specifically detects an extracellular stimulus (a photon, pheromone, odorant,
hormone or neurotransmitter) while the effectors (e.g., adenyl cyclase), which
act to generate one or more intracellular messengers, are less numerous. In
mammals, G protein α, β and γ polypeptides are encoded by at least 16, 4
and 7 genes, respectively. Most interest in G proteins has been focused on
their α subunits, since these proteins bind and hydrolyze GTP and most obvi-
ously regulate the activity of the best studied effectors. Evidence, however,
has established an important regulatory role for the βγ subunits. It is becom-
ing increasingly clear that different G protein complexes expressed in differ-
ent tissues carry structurally distinct members of the γ, as well as the α and
β, subunits and that preferential associations between members of subunit
families increase G protein functional diversity.
APPLICATIONS
Gγ 3 (K-20) is recommended for detection of Gγ 3 of broad origin by Western
blotting, immunoprecipitation [1–2 µg per 100–500 µg of total protein (1 ml
of cell lysate)], immunofluorescence (starting dilution 1:50, dilution range
1:50-1:500) and solid phase ELISA (starting dilution 1:30, dilution range
1:30-1:3000).
Suitable for use as control antibody for Gγ 3 siRNA (h): sc-41776 and Gγ 3
siRNA (m): sc-41777.
Molecular Weight of Gγ 3: 3-7 kDa.
Positive Controls: mouse cerebellum extract: sc-2403 or rat brain extract:
sc-2392.
DATA
REFERENCES
1. Gautam, N., et al. 1990. G protein diversity is increased by associations
with a variety of γ subunits. Proc. Natl. Acad. Sci. USA 87: 7973-7977.
2. Simon, M.I., et al. 1991. Diversity of G proteins in signal transduction.
Science 252: 802-808.
3. von Weizsäcker, E., et al. 1992. Diversity among the β subunits of het-
erotrimeric GTP-binding proteins: characterization of a novel β-subunit
cDNA. Biochem. Biophys. Res. Comm. 183: 350-356.
4. Kleuss, C., et al. 1992. Different β-subunits determine G protein interac-
tion with transmembrane receptors. Nature 358: 424-426.
5. Blank, J.L., et al. 1992. Activation of cytosolic phosphoinositide phospholi-
pase C by G protein βγ subunits. J. Biol. Chem. 267: 23069-23075.
CHROMOSOMAL LOCATION
Genetic locus: GNG3 (human) mapping to 11p11; Gng3 (mouse) mapping to
19 A.
SOURCE
Gγ 3 (K-20) is an affinity purified rabbit polyclonal antibody raised against a
peptide mapping at the N-terminus of Gγ 3 of bovine origin.
PRODUCT
Each vial contains 200 µg IgG in 1.0 ml of PBS with < 0.1% sodium azide
and 0.1% gelatin.
Blocking peptide available for competition studies, sc-375 P, (100 µg peptide
in 0.5 ml PBS containing < 0.1% sodium azide and 0.2% BSA).
STORAGE
Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of
shipment. Non-hazardous. No MSDS required.
46 K -
30 K -
22 K -
14 K -
7K-
3K-
< Gγ 3
34 K –
23 K –
< Gγ 3
Gγ 3 (K-20): sc-375. Western blot analysis of Gγ 3
expression in rat brain extract.
Gγ 3 (K-20): sc-375. Western blot analysis of Gγ 3
expression in mouse brain tissue extract.
SELECT PRODUCT CITATIONS
1. Kowluru, A., et al. 1997. Glucose activates the carboxyl methylation
of γ subunits of trimeric GTP-binding proteins in pancreatic β cells.
Modulation in vivo by calcium, GTP, and pertussis toxin. J. Clin. Invest.
100: 1596-1610.
2. Macrez-Lepretre, N., et al. 1997. G protein heterotrimer Gα 13/β 1/γ 3
couples the angiotensin AT1A receptor to increases in cytoplasmic Ca2+
in rat portal vein myocytes. J. Biol. Chem. 272: 10095-10102.
3. Macrez, N., et al. 1997. A βγ dimer derived from G13 transduces the
angiotensin AT1 receptor signal to stimulation of CA2+ channels in rat
portal vein myocytes. J. Biol. Chem. 272: 23180-23185.
4. Schuller, U., et al. 2001. Developmental expression of heterotrimeric G-
proteins in the murine cerebellar cortex. Histochem. Cell Biol. 116: 149-159.
5. Sokolov, M., et al. 2004. Phosducin facilitates light-driven transducin
translocation in rod photoreceptors: evidence from the phosducin knockout
mouse. J. Biol. Chem. 279: 19149-19156.
6. Lobanova, E.S., et al. 2008 . Transducin γ-subunit sets expression levels
of α- and β-subunits and is crucial for rod viability. J. Neurosci. 28:
3510-3520.
RESEARCH USE
For research use only, not for use in diagnostic procedures.
Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com

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